Three Members of a Clan at another Bench are hitting the Fourth Member with their Lab Notebooks. You go over to their Bench to investigate. “Look at

5. Three Members of a Clan at another Bench are hitting the Fourth Member
with their Lab Notebooks. You go over to their Bench to investigate.
“Look at these Plates!” shouts Biff. You look at the Plates. Each appears to be
half-filled with Top Agar, like a Slant on a Plate. And in each Case the Top
Agar seems to be covering about Half (~50%) of the Surface of its Bottom
Agar Plate. What’s going on?
“Ask Alfonso!” shouts Bucky. You deduce that Alfonso is the Target of their
Wrath. “Tell him!” shouts Buffy, giving Alfonso another Whack. You confiscate
Buffy’s Lab Notebook. You ask this Clan a Series of Questions:
• “How much Top Agar did you add to each of the small Test Tubes in the
Heating Block?” Alfonso says approximately 1.5 ml.
• “How much of each Bacteriophage Dilution did you add to each of the small
Test Tubes when you removed them from the Heating Block?” Buffy
answers that she added exactly 100 μl.
• “How much E. coli did you add to each of the small Test Tubes after the
Bacteriophage Dilution was added?” Bucky says he added exactly 200 μl.
• “Did you vortex each of the small Test Tubes after the Bacteriophage and the
E. coli were added and did you pour all 1.5 ml of Top Agar into the Bottom
Agar Plate?” Alfonso answers Yes.
• “How did you create these Half Top Agars?” Alfonso says he poured the
Contents of each Top Agar Tube into the Bottom of each Agar Plate.
That’s it. No Mad Hatter Tea Party with any of these Plates. He just poured
the Contents of each Tube of Top Agar over one Half of the appropriate
Bottom Agar Plate and allowed the Top Agar to solidify.
• “What are your Plaque Counts for each Plate of these Top Agar Slants?”
10-4 10-5 10-6 10-7 10-8 10-9
TNTC TNTC TNTC TNTC 423 42
You look at these Plaque Counts, you look at the Plates, and finally
you tell Alfonso, Biff, Buckey, Buffy that their Phage Titer is —
A. 2.1 x 1010 pfu/ml.
____________________________
B. 4.2 x 1010 pfu/ml.
____________________________
C. 8.5 x 1010 pfu/ml.
____________________________
D. 1.7 x 1011 pfu/ml.
Hi normally the top agar should be 3ml instead of 1.5ml, and normally we would do Mad Tea Party to evenly distribute the top agar over the surface of the bottom agar. Would this have any effect on the result? The plaque count should be 30-300 so only 42 is valid.
Three Members of a Clan at another Bench are hitting the Fourth Memberwith their Lab Notebooks. You go over to their Bench to investigate. “Look at these Plates!” shouts Biff. You look at the Plates. Each appears to behalf-filled with Top Agar, like a Slant on a Plate. And in each Case the TopAgar seems to be covering about Half (~50%) of the Surface of its BottomAgar Plate. What’s going on? “Ask Alfonso!“ shouts Bucky. You deduce that Alfonso is the Target of theirWrath. “Tell him!" shouts Buffy, giving Alfonso another Whack. You confiscateBuffy’s Lab Notebook. You ask this Clan a Series of Questions: – “How much Top Agar did you add to each of the small Test Tubes in theHeating Block?” Alfonso says approximately 1.5 ml. – “How much of each Bacteriophage Dilution did you add to each of the smallTest Tubes when you removed them from the Heating Block?” Buffy answers that she added exactly 100 pl. – “How much E. coli did you add to each of the small Test Tubes after theBacteriophage Dilution was added?” Bucky says he added exactly 200 ul. – “Did you vortex each of the small Test Tubes after the Bacteriophage and the E. coli were added and did you pour all 1.5 H11 of Top Agar into the BottomAgar Plate?” Alfonso answers Yes. – “How did you create these Half Top Agars?” Alfonso says he poured theContents of each Top Agar Tube into the Bottom of each Agar Plate.That’s it. No Mad Hatter Tea Party with any of these Plates. He just poured the Contents of each Tube of Top Agar over one Half of the appropriateBottom Agar Plate and allowed the Top Agar to solidify. – “What are your Plaque Counts for each Plate of these Top Agar Slants?”10’4 10-5 10-6 10“ 10-8 10’9 TNTC TNTC TNTC TNTC 423 42 You look at these Plaque Counts, you look at the Plates, and finallyyou tell Alfonso, Biff, Buckey, Buffy that their Phage Titer is — A. 2.1 x 10*10 pfulml.B. 4.2 x 1D10 pfufml.C. 8.5 x1010 pfulml. D. 1.7 x1011 pfufml.
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